Bear in mind that a very concentrated sample will produce a quick 13C spectrum, but may result in a broadened 1H lineshape. When the amount of material is doubled, the resultant signal will be doubled.
This amount of material will allow you to obtain a 1H spectrum in a few minutes or a 13C spectrum in 20-60 minutes. Typical 13C spectra require 50-100 mg of material. For small molecules (less than 1000 g/mol), typical 1H NMR spectra require 5-25 mg of material.Additional preparation procedures for large molecules, polymers, biomolecules, inorganic molecules, etc. This tutorial covers the basic preparation for most small-molecule organic compounds. Purchase of tubes and solvents is discussed below. All users must supply their own NMR tubes and solvents. This page covers a variety of considerations for good preparation of NMR samples. ( Download this page in PDF format for printing)
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